p53 Gene Mutations in Esophageal Cancer Detected by Polymerase Chain Reaction Single-strand Conformation Polymorphism Analysis
نویسندگان
چکیده
منابع مشابه
Correlation of p53 over-expression and alteration in p53 gene detected by polymerase chain reaction-single strand conformation polymorphism in adenocarcinoma of gastric cancer patients from India.
AIM To study the alterations in p53 gene among Indian gastric cancer patients and to correlate them with the various clinicopathological parameters. METHODS A total of 103 gastric cancer patients were included in this study. The p53 alterations were studied by both immunohistochemical method as well as polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) analysis. We...
متن کاملRole of p53 mutations in endocrine tumorigenesis: mutation detection by polymerase chain reaction-single strand conformation polymorphism.
To elucidate the molecular basis for endocrine tumorigenesis, p53 mutations in human endocrine tumors were analyzed by using polymerase chain reaction-single strand conformation polymorphism. Exons 5 through 10 of the p53 gene were studied in genomic DNAs from 134 primary endocrine tumors and 6 human endocrine cancer-derived cell lines. Mutations were detected and identified in 4 endocrine tumo...
متن کاملallelic polymorphism of makoei sheep calpastatin gene identified by polymerase chain reaction and single strand conformation polymorphism
calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study, the polymorphism of the cast gene of makoei sheep was investigated by polymerase chain reaction and single strand conformation polymorphism technique (pcr–sscp). genomic dna was extracted from whole blood samples collected from 100 sheep. a 622 bp cast exon 1 segmen...
متن کاملDetection of frequent p53 gene mutations in primary gastric cancer by cell sorting and polymerase chain reaction single-strand conformation polymorphism analysis.
Mutations of the p53 gene were investigated after tumor cell enrichment by cell sorting based on differences in DNA content and polymerase chain reaction single-strand conformation polymorphism analysis in 24 surgical specimens of primary gastric cancer. p53 mutations were detected in exons 4-8 in 64% (9 of 14) of aneuploid tumors but in none of 10 diploid tumors examined. Four of five tumors c...
متن کاملEffect of Non-Tumor Cell Contamination on Detection of p53 Gene Mutations in Human Gastric Cancer Cells by Polymerase Chain Reaction Single-Strand Conformation Polymorphism Analysis
BACKGROUND We have previously studied p53 gene mutations in 25 primary gastric cancer tissues by polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) analysis for exon 4-8 and immunohistochemical staining with anti-p53 antibody. In four cases, the discrepancy of the results was observed between the two methods. In one case positive by PCR-SSCP but negative by immunohisto...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Cancer Research
سال: 1992
ISSN: 0910-5050
DOI: 10.1111/j.1349-7006.1992.tb00125.x